Application
Western blot analysis of hnRNP U expression in non-transfected: (A) and human hnRNP U transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.| Product Name | hnRNP U Antibody (H-94) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 731-824 of hnRNP U of human origin |
| Modification Notes | RNA polymerase II transcripts are complexed with hnRNP (heterogeneous nuclear ribonucleoprotein) proteins, which are involved in several aspects of hnRNA maturation and transport. The hnRNP particle U (also designated SP120 and SAF-A for scaffold attachment factor) is an abundant nucleoplasmic phosphoprotein and the largest of the major hnRNP proteins. hnRNP U is specifically involved in pre-mRNA processing and is directly bound to both RNA and DNA. Specifically, hnRNP U has a high affinity to the SAR (scaffold attachment region) of DNA . hnRNP U also functions as an RNA polymerase elongation inhibitor by inhibiting TFIIH-mediated phosphorylation of the carboxy-terminal domain of Pol II. Identical to GRIP120, hnRNP U also associates with glucocorticoid receptors to inhibit glucocorticoid induction. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of hnRNP U of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 731-824 of hnRNP U of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 731-824 of hnRNP U of human origin |
| Entrez Gene | 3192 |
|---|
Application
Western blot analysis of hnRNP U expression in non-transfected: (A) and human hnRNP U transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing nuclear staining.| Application Notes | recommended for detection of hnRNP U of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
