Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear localization.| Product Name | RAG-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 744-1043 mapping at the C-terminus of RAG-1 of human origin |
| Modification Notes | Immunoglobulin (Ig) and the T cell receptor are the receptors of B and T lymphocytes and are encoded in multiple germ line DNA segments, V, D and J, that are rearranged during lymphocyte development. This is the only known example of site specific recombination in vertebrate genes. Several genes are essential for V(D)J rearrangement. The recombination activator genes RAG1 and RAG2 were originally identified on the basis of their ability to activate rearrangement of an exogenous recombinational substrate in fibroblasts; moreover, both genes are required for this activity. It is yet to be resolved as to whether RAG1 and RAG2 encode components of the V(D)J recombinase itself or regulatory proteins that potentiate V(D)J recombination. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RAG-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 744-1043 mapping at the C-terminus of RAG-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 744-1043 mapping at the C-terminus of RAG-1 of human origin |
| Entrez Gene | 5896 |
|---|
Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear localization.| Application Notes | recommended for detection of RAG-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
