Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing membrane staining of cells in white pulp.Application
Western blot analysis of CD74 expression in non-transfected: (A) and mouse CD74 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD74 expression in BJAB whole cell lysate.Application
Western blot analysis of CD74 expression in human PBL whole cell lysate.Application
Western blot analysis of CD74 expression in non-transfected: (A) and mouse CD74 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD74 expression in non-transfected: (A) and mouse CD74 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of CD74 expression in non-transfected control (A) and CD74 siRNA transfected (B) HeLa cells. Blot probed with CD74 (FL-296): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of CD74 expression in J774A.1 (A), RAW 264.7 (B), IB4 (C), NFS-5 C-1 (D) and I-11.15 (E) whole cell lysates.Application
Western blot analysis of CD74 expression in NAMALWA whole cell lysate.| Product Name | CD74 Antibody (FL-296) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 17-296 of CD74 of human origin |
| Modification Notes | The human histocompatibility leukocyte antigen (HLA) class II-associated invariant chain is composed of at least four polypeptides (1–3). One of these polypeptide chains is expressed as a membrane-bound subunit and has been designated CD74 (4,5). The loading of peptide onto the class II MHC protein (MHC II) appears to be regulated by CD74, which associates with MHC II during its migration to the endosomal compartment, where class II binds pep-tide (1). CD74 is expressed by cells of both T lymphocyte and B lymphocyte lineages (6). In fact, CD74 is broadly expressed in normal B lymphocytes, regardless of their histocompatibility leukocyte antigen (HLA) phenotype, while a subset of peripheral T lymphocytes that are MHC II negative do not express CD74 (6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CD74 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 17-296 of CD74 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 17-296 of CD74 of human origin |
| Entrez Gene | 972 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human spleen tissue showing membrane staining of cells in white pulp.
Application
Western blot analysis of CD74 expression in non-transfected: (A) and mouse CD74 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD74 expression in BJAB whole cell lysate.
Application
Western blot analysis of CD74 expression in human PBL whole cell lysate.
Application
Western blot analysis of CD74 expression in non-transfected: (A) and mouse CD74 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD74 expression in non-transfected: (A) and mouse CD74 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of CD74 expression in non-transfected control (A) and CD74 siRNA transfected (B) HeLa cells. Blot probed with CD74 (FL-296): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of CD74 expression in J774A.1 (A), RAW 264.7 (B), IB4 (C), NFS-5 C-1 (D) and I-11.15 (E) whole cell lysates.
Application
Western blot analysis of CD74 expression in NAMALWA whole cell lysate.| Application Notes | recommended for detection of CD74 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
