Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining of epidermal cells.Application
Western blot analysis of pan-Cytokeratin expression in non-transfected 293T: (A), human Cytokeratin 10 transfected 293T: (B) and HeLa (C) whole cell lysate.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cyto-skeletal localization.Application
Western blot analysis of Cytokeratin expression in HeLa whole cell lysate.| Product Name | pan-Cytokeratin Antibody (H-240) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 181-420 mapping to an internal region of Cytokeratin 10 of human origin |
| Modification Notes | Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue (1,2). Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells (3,4). Cytokeratins have been found to be useful markers of tissue differentiation which is directly applicable to the characterization of malignant tumors (5,6). For example, cytokeratins 10 and 13 are expressed highly in a subset of squamous cell carcinomas while cytokeratin 18 is expressed in a majority of adenocarcinomas and basal cell carcinomas (5-6). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| broadly reactive with Cytokeratin family members of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 181-420 mapping to an internal region of Cytokeratin 10 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 181-420 mapping to an internal region of Cytokeratin 10 of human origin |
| Entrez Gene | 3858 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining of epidermal cells.
Application
Western blot analysis of pan-Cytokeratin expression in non-transfected 293T: (A), human Cytokeratin 10 transfected 293T: (B) and HeLa (C) whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cyto-skeletal localization.
Application
Western blot analysis of Cytokeratin expression in HeLa whole cell lysate.| Application Notes | broadly reactive with Cytokeratin family members of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
