Application
Immunofluorescence staining of methanol-fixed Hs68 cells showing cytoplasmic and extracellular localization.Application
Western blot analysis of COL5A1 expression in CCD-1064Sk (A) and Hs68 (B) whole cell lysates.| Product Name | COL5A1 Antibody (H-200) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 251-450 of Collagen α1 Type V of human origin |
| Modification Notes | The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Collagen α1 Type V of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 251-450 of Collagen &alpha 1 Type V of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 251-450 of Collagen α1 Type V of human origin |
| Entrez Gene | 1289 |
|---|
Application
Immunofluorescence staining of methanol-fixed Hs68 cells showing cytoplasmic and extracellular localization.
Application
Western blot analysis of COL5A1 expression in CCD-1064Sk (A) and Hs68 (B) whole cell lysates.| Application Notes | recommended for detection of Collagen α1 Type V of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
