Application
Western blot analysis of CD45 expression in Jurkat (A) and CCRF-CEM (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing membrane localization.| Product Name | CD45 Antibody (H-230) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1075-1304 mapping at the C-terminus of CD45 of human origin |
| Modification Notes | CD45 has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45 are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5 and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45, while the intracellular domain is conserved. CD45 functions as a phosphotyrosine phosphatase, a vital component for efficient tyrosine phosphorylation induction by the TCR/CD3 complex. The tyrosine phosphatase activity of CD45 is contained within the conserved intracellular domain. Src and Syk family protein tyrosine kinases are utilized by the TCR/CD3 complex to initiate signaling cascades. Several members of these two families, including Lck, Fyn and ZAP-70, have been implicated as physiological substrates of CD45. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD45 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1075-1304 mapping at the C-terminus of CD45 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1075-1304 mapping at the C-terminus of CD45 of human origin |
| Entrez Gene | 5788 |
|---|
Application
Western blot analysis of CD45 expression in Jurkat (A) and CCRF-CEM (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing membrane localization.| Application Notes | recommended for detection of CD45 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
