Application
Western blot analysis of CD3-ε expression in non-transfected: (A) and human CD3-ε transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing membrane localization.Application
Western blot analysis of CD3- expression in HuT 78 (A) and H9 (B) whole cell lysates.Application
Western blot analysis of CD3-ε expression in Jurkat (A) and CCRF-CEM (B) whole cell lysates.| Product Name | CD3-ε Antibody (FL-207) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-207 representing full length CD3-ε of human origin |
| Modification Notes | The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (CD3-© and CD3-é), a heterodimer of delta and epsilon chains (CD3-∂ and CD3-é) and a homodimer of two zeta chains (CD3-Ω) or a heterodimer of zeta and eta chains (CD3-Ω and CD3-h). CD3-Ω and CD3-h are encoded by the same gene, but differ in their carboxyl-terminal ends due to an alternative splicing event. CD3-©, CD3-é and CD3-∂ each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, CD3-Ω contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to CD3-Ω ITAMs has been solved. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of CD3-ε of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-207 representing full length CD3-&epsilon of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-207 representing full length CD3-ε of human origin |
| Entrez Gene | 916 |
|---|
Application
Western blot analysis of CD3-ε expression in non-transfected: (A) and human CD3-ε transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing membrane localization.
Application
Western blot analysis of CD3- expression in HuT 78 (A) and H9 (B) whole cell lysates.
Application
Western blot analysis of CD3-ε expression in Jurkat (A) and CCRF-CEM (B) whole cell lysates.| Application Notes | recommended for detection of CD3-ε of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
