Application
Western blot analysis of Homer-1b/c expression in IMR-32 whole cell lysate.Application
Western blot analysis of Homer expression in non-transfected: (A) and human Homer transfected: (B) 293T whole cell lysates and rat brain tissue extract (C).Application
Western blot analysis of Homer-1b/c expression in non-transfected: (A) and human Homer transfected: (B) 293 whole cell lysates and mouse brain tissue extract (C).Application
Western blot analysis of Homer-1b/c expression in mouse (A) and rat (B) brain tissue extracts.Application
Immunofluorescence staining of methanol-fixed A-10 cells showing cytoplasmic and membrane localization.| Product Name | Homer-1b/c Antibody (H-174) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 181-354 of Homer-1b of human origin |
| Modification Notes | Homer family proteins, also designated Vesl (for VASP/Ena-related gene upregulated during seizure) and LTP, are immediate early gene products that bind to group 1 metabotropic glutamate receptors (mGluRs), proteins involved in triggering intracellular calcium release. Unlike Homer-1a, the prototype member of the Homer family, other Homer family members (Homer-1b and -1c, Homer-2a, -2b and -2c and Homer-3) are constitutively expressed and contain a coiled-coil (CC) domain that mediates self-multimerization. Homer-1a is enriched at excitatory synapses, does not multimerize and appears to block the association of mGluRs to CC-Homer proteins. Homer proteins have also been shown to link mGluRs with the inositol triphosphate receptors (IP3R). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Homer-1b and Homer-1c of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 181-354 of Homer-1b of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 181-354 of Homer-1b of human origin |
| Entrez Gene | 9456 |
|---|
Application
Western blot analysis of Homer-1b/c expression in IMR-32 whole cell lysate.
Application
Western blot analysis of Homer expression in non-transfected: (A) and human Homer transfected: (B) 293T whole cell lysates and rat brain tissue extract (C).
Application
Western blot analysis of Homer-1b/c expression in non-transfected: (A) and human Homer transfected: (B) 293 whole cell lysates and mouse brain tissue extract (C).
Application
Western blot analysis of Homer-1b/c expression in mouse (A) and rat (B) brain tissue extracts.
Application
Immunofluorescence staining of methanol-fixed A-10 cells showing cytoplasmic and membrane localization.| Application Notes | recommended for detection of Homer-1b and Homer-1c of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
