Application
Western blot analysis of Gγ 7 expression in mouse brain tissue extract.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes and bile duct cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.Application
Western blot analysis of Gγ7 expression in rat brain extract.| Product Name | Gγ 7 Antibody (S-14) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (i.e. a photon, pheromone, odorant, hormone or neurotransmitter), while the effectors (e.g. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein å, ∫ and © polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Evidence, however, has established an important regulatory role for the ∫© subunits. It is becoming increasingly clear that different G protein complexes expressed in different tissues carry structurally distinct members of the © as well as the å and ∫ subunits, and that preferential associations between members of subunit families increase G protein functional diversity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,IHC,ELISA |
| recommended for detection of G&gamma 7 in a broad range of mammalian species by WB, IP, IF, IHC(P) and ELISA | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of G&gamma 7 of bovine origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of Gγ 7 of bovine origin |
Application
Western blot analysis of Gγ 7 expression in mouse brain tissue extract.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human liver tissue showing cytoplasmic staining of hepatocytes and bile duct cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.
Application
Western blot analysis of Gγ7 expression in rat brain extract.| Application Notes | recommended for detection of G&gamma 7 in a broad range of mammalian species by WB, IP, IF, IHC(P) and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
