Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Gγ 3 expression in mouse brain tissue extract.Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.Application
Western blot analysis of Gγ 3 expression in rat brain extract.| Product Name | Gγ 3 Antibody (K-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of Gγ 3 of bovine origin |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (i.e. a photon, pheromone, odorant, hormone or neurotransmitter), while the effectors (e.g. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein å, ∫ and © polypeptides are encoded by at least 16, 4 and 7 genes, respectively. Most interest in G proteins has been focused on their a subunits, since these proteins bind and hydrolyze GTP and most obviously regulate the activity of the best studied effectors. Evidence, however, has established an important regulatory role for the ∫© subunits. It is becoming increasingly clear that different G protein complexes expressed in different tissues carry structurally distinct members of the © as well as the å and ∫ subunits, and that preferential associations between members of subunit families increase G protein functional diversity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Gγ 3 in a broad range of mammalian species by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of G&gamma 3 of bovine origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of Gγ 3 of bovine origin |
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Gγ 3 expression in mouse brain tissue extract.
Application
Immunofluorescence staining of methanol-fixed SK-N-SH cells showing cytoplasmic localization.
Application
Western blot analysis of Gγ 3 expression in rat brain extract.| Application Notes | recommended for detection of Gγ 3 in a broad range of mammalian species by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
