Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islet of langerhans and exocrine glandular cells.Application
Western blot analysis of Sos 1 expression in K-562 whole cell lysate.Application
Western blot analysis of Sos 1 expression in non-transfected 293T: (A), human Sos 1 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Sos 1 siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Sos 1 (C-23): .Application
Immunofluorescence staining of methanol-fixed HeLa cells.Application
Western blot analysis of Sos 1 p170 expression in NIH/3T3 (A) and KNRK (B) whole cell lysates.| Product Name | Sos 1 Antibody (C-23) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Sos 1 of human origin |
| Modification Notes | The superfamily of GTP-binding proteins, of which Ras proteins are prototypes, has been implicated in a broad range of biological activities. Studies have identified a family of guanine nucleotide-releasing factors (GRFs) that activate Ras in mammalian cells and an “adapter” protein (Sem 5/GRB2) that appears to mediate the interaction of GRFs with activated receptor molecules (1-3). Ras-GRF p140 promotes nucleotide exchange on ras p21s but not on other members of the Ras gene superfamily. In addition, three mammalian homologs of the Drosophila Ras-GRF, son of sevenless (Sos), have been described (4-6). These include two from mouse, m Sos 1 and m Sos 2, and one from human, h Sos. Vav p95 has been reported to function as a GRF in activation of Ras by the T cell receptor and has been reported to have a domain similar to that of Dbl p115, which is a GRF specific for CDC42Hs. Subsequent to activation, Ras appears to interact with Raf, thereby activating the MAP kinase phosphorylation pathway (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Sos 1 p170 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Sos 1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Sos 1 of human origin |
| Entrez Gene | 6654 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of Islet of langerhans and exocrine glandular cells.
Application
Western blot analysis of Sos 1 expression in K-562 whole cell lysate.
Application
Western blot analysis of Sos 1 expression in non-transfected 293T: (A), human Sos 1 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed, control HeLa (A) and Sos 1 siRNA silenced HeLa (B) cells showing diminished cytoplasmic staining in the siRNA silenced cells. Cells probed with Sos 1 (C-23): .
Application
Immunofluorescence staining of methanol-fixed HeLa cells.
Application
Western blot analysis of Sos 1 p170 expression in NIH/3T3 (A) and KNRK (B) whole cell lysates.| Application Notes | recommended for detection of Sos 1 p170 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
