Application
Western blot analysis of Syntaxin expression in mouse cerebellum (A), mouse brain (B) and rat brain (C) tissue extracts.Application
Immunofluorescence staining of methanol-fixed PC-12 cells showing membrane staining.Application
Western blot analysis of Syntaxin expression in mouse (A) and rat (B) brain extracts.| Product Name | Syntaxin Antibody (H-222) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 3-225 of Syntaxin 1A of human origin |
| Modification Notes | Correct vesicular transport is essential to the survival of eukaryotic cells. This process is determined by specific pairing of vesicle-associated SNAREs (v-SNAREs) with those on the target membrane (t-SNAREs). This complex then recruits soluble NSF attachment proteins (SNAPs) and N-ethylmaleimide-sensitive factor (NSF) to form the highly stable SNAP receptor (SNARE) complex. The formation of a SNARE complex pulls the vesicle and target membrane together and may provide the energy to drive fusion of the lipid bilayers. SNAPs, including a- and g-SNAP, are cytoplasmic proteins that bind to a membrane receptor complex composed of VAMP, SNAP 25 and Syntaxin. Syntaxins, a family of proteins involved in the fusion of synaptic vesicles with the plasma membrane, display broad tissue distribution and contain C-terminal hydrophobic domains that direct themselves to their respective intracellular compartments. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of a broad range of Syntaxin family members of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 3-225 of Syntaxin 1A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 3-225 of Syntaxin 1A of human origin |
| Entrez Gene | 6811 |
|---|
Application
Western blot analysis of Syntaxin expression in mouse cerebellum (A), mouse brain (B) and rat brain (C) tissue extracts.
Application
Immunofluorescence staining of methanol-fixed PC-12 cells showing membrane staining.
Application
Western blot analysis of Syntaxin expression in mouse (A) and rat (B) brain extracts.| Application Notes | recommended for detection of a broad range of Syntaxin family members of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
