Application
Western blot analysis of GβL expression in non-transfected (A) and human GβL transfected (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Product Name | GβL Antibody (V-24) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of GβL of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | Heterotrimeric G proteins function to relay information from cell surface receptors to intracellular effectors. Each of a very broad range of receptors specifically detects an extracellular stimulus (i.e. a photon, pheromone, odorant, hormone or neurotransmitter), while the effectors (e.g. adenyl cyclase), which act to generate one or more intracellular messengers, are less numerous. In mammals, G protein å, ∫ and © polypeptides are encoded by at least 16, 4 and 7 genes, respectively. G∫L (G protein beta subunit-like), also known as LST8, POP3 or WAT1, is a 326 amino acid protein that localizes to the cytoplasm and contains 7 WD repeats. Expressed in a variety of tissues with highest expression in heart, kidney and skeletal muscle, G∫L functions as a component of the TORC1 and TORC2 complex and plays an important role in cell growth response to environmental stimuli. Four isoforms of G∫L exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of G&beta L of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of GβL of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 64223 |
|---|
Application
Western blot analysis of GβL expression in non-transfected (A) and human GβL transfected (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
