Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of Axin expression in K-562 (A), HeLa (B) and SK-N-SH (C) whole cell lysates and Hep G2 (D) and K-562 (E) nuclear extracts.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.Application
Western blot analysis of Axin expression of K-562 (A), HeLa (B) and SK-N-SH (C) whole cell lysates and HeLa nuclear extract (D).| Product Name | Axin Antibody (H-98) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 646-743 of Axin of human origin |
| Modification Notes | ∫-catenin is a component of both the cadherin cell adhesion system and the Wnt signaling pathway. Wnt signaling increases the amount of ∫-catenin by preventing its ubiquitination and degradation, allowing its direct interaction with transcription factors of the lymphoid enhancer factor/T cell factor family, and modulation of gene expression. Axin is involved in the degradation of ∫-catenin by acting as a scaffold to form a complex between ∫-catenin, adenomatous polyposis coli (APC) and GSK-3∫. APC, which is phosphorylated by GSK-3∫, induces degradation of ∫-catenin, thus inhibiting Wnt signal transduction. Conductin is 45% identical to Axin and appears to play a similar role to Axin in the Wnt signaling pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Axin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 646-743 of Axin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 646-743 of Axin of human origin |
| Entrez Gene | 8312 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing cytoplasmic localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of Axin expression in K-562 (A), HeLa (B) and SK-N-SH (C) whole cell lysates and Hep G2 (D) and K-562 (E) nuclear extracts.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.
Application
Western blot analysis of Axin expression of K-562 (A), HeLa (B) and SK-N-SH (C) whole cell lysates and HeLa nuclear extract (D).| Application Notes | recommended for detection of Axin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
