Application
Western blot analysis of twist expression in Jurkat whole cell lysate.| Product Name | twist Antibody (L-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of twist of mouse, rat, human, canine and zebrafish origin by WB, IP and ELISA |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | Members of the myogenic determination family are basic helix-loop-helix (bHLH) proteins that can be separated into two classes. Class A proteins include the ubiquitously expressed E-box binding factors E12/E47, ITF2 and HEB (BETA1 or HTF4). Class B proteins such as MyoD, myogenin and NeuroD (BETA2) are transiently expressed and exhibit a much more limited tissue distribution. Class A proteins heterodimerize with class B proteins to activate DNA transcription. Working in opposition to these positively acting factors are a specialized group of proteins that function as dominant negative regulators. Muscle tissue is derived from a subset of cells originating from the embryonic mesoderm. The novel basic helix-loop-helix (bHLH) transcription factor twist is a putative regulator of mesodermal differentiation and myogenesis. Twist is expressed throughout the epithelial somite but not in the myotome. Twist requires dimerization with the E proteins and inhibits myogenic regulatory factors. It has been implicated as regulator of the temporal and spatial formation of myotomes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| WB:1:100~1:500 IP:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of twist of mouse rat human canine and zebrafish origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of twist of mouse, rat, human, canine and zebrafish origin by WB, IP and ELISA |
| Entrez Gene | 7291 |
|---|
Application
Western blot analysis of twist expression in Jurkat whole cell lysate.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
