Application
Western blot analysis of GnT-I expression in Y79 whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.Application
Western blot analysis of GnT-I expression in Jurkat whole cell lysate.| Product Name | GnT-I Antibody (Y-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of GnT-I of human origin by WB, IP, IF, IHC(P) and ELISA |
| Modification Notes | GnT-I (a-1,3-mannosyl-glycoprotein 2-b-N acetylglucosaminyltransferase, GlcNAc-T I, MGAT1) catalyzes the first step in the conversion of oligomannose-type N-glycans to N-acetyl-lactosamine- and hybrid-type N-glycans. GnT-I is a 445 amino acid type II membrane protein that localizes to the Golgi and is essential for normal embryogenesis. GnT-I is expressed ubiquitously and exists as multiple variants that encode the same functional protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of GnT-I of human origin by WB IP IF IHC(P) and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of GnT-I of human origin by WB, IP, IF, IHC(P) and ELISA |
| Entrez Gene | 4245 |
|---|
Application
Western blot analysis of GnT-I expression in Y79 whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue showing cytoplasmic localization.
Application
Western blot analysis of GnT-I expression in Jurkat whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 IF:1:200~1:500 IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
