Application
Western blot analysis of NT5C3 expression in Jurkat whole cell lysate.Application
Western blot analysis of NT5C3 expression in Hep G2 whole cell lysate.| Product Name | NT5C3 Antibody (T-25) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of NT5C3 of human origin by WB, IP and ELISA |
| Modification Notes | Nucleotidases are hydrolytic enzymes that catalyze the hydrolysis of nucleotides into phosphates and nucleosides. NT5C3 (5'-nucleotidase, cytosolic III), also known as P5N1 or UMPH1, is a 336 amino acid protein that exists as multiple alternatively spliced isoforms which localize to either the cytoplasm or the endoplasmic reticulum. Expressed in an isoform-specific manner in lymphocytes and reticulocytes, NT5C3 belongs to the pyrimidine 5'-nucleotidase family and exists as a monomer which acts as both a nucleotidase and a phosphotransferase, effectively catalyzing the conversion of a 5'-ribonucleotide to a ribonucleoside and a free phosphate. Defects in the gene encoding NT5C3 are the cause of P5N deficiency, an autosomal recessive disorder that is associated with hemolytic anemia and is characterized by lead poisoning and learning difficulties. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of NT5C3 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of NT5C3 of human origin by WB, IP and ELISA |
| Entrez Gene | 51251 |
|---|
Application
Western blot analysis of NT5C3 expression in Jurkat whole cell lysate.
Application
Western blot analysis of NT5C3 expression in Hep G2 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
