Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear staining of pneumocytes.Application
Western blot analysis of SIRT1 expression in non-transfected: (A) and human SIRT1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SIRT1 expression in F9 whole cell lysate.Application
Immunofluorescence staining of methanol-fixed MES-SA/Dx5 cells showing nuclear localization.Application
Western blot analysis of SIRT1 expression in K-562 nuclear extract.Application
Western blot analysis of SIRT1 expression in MES-SA/Dx5 whole cell lysate.| Product Name | SIRT1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 448-747 mapping at the C-terminus of sirtuin 1 of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | The silent information regulator (SIR2) family of genes are highly-conserved from prokaryotes to eukaryotes and are involved in diverse processes, including transcriptional regulation, cell cycle progression, DNA-damage repair and aging. In S. cerevisiae, Sir2p deacetylates histones in an NAD-dependent manner, which regulates silencing at the telomeric, rDNA and silent mating-type loci. Sir2p is the founding member of a large family, designated sirtuins, which contain a conserved catalytic domain. The human homologs, which include SIRT1-7, are divided into four main branches: SIRT1-3 are class I, SIRT4 is class II, SIRT5 is class III and SIRT6-7 are class IV. SIRT1 has the closest homology to the yeast Sir2p and is widely expressed in fetal and adult tissues. SIRT1 is highly expressed in heart, brain and skeletal muscle, with low expression in lung and placenta. SIRT1 regulates the p53-dependent DNA damage response pathway by binding to and deacetylating p53, specifically at Lys 382. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 448-747 mapping at the C-terminus of sirtuin 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 448-747 mapping at the C-terminus of sirtuin 1 of human origin |
| Entrez Gene | 23411 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing nuclear staining of pneumocytes.
Application
Western blot analysis of SIRT1 expression in non-transfected: (A) and human SIRT1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SIRT1 expression in F9 whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed MES-SA/Dx5 cells showing nuclear localization.
Application
Western blot analysis of SIRT1 expression in K-562 nuclear extract.
Application
Western blot analysis of SIRT1 expression in MES-SA/Dx5 whole cell lysate.| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
