Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing membrane localization.Application
Western blot analysis of glypican-3 expression in Hep G2 whole cell lysate.Application
Western blot analysis of human recombinant glypican-3 fusion protein.| Product Name | glypican-3 Antibody (H-162) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 303-464 mapping near the C-terminus of glypican-3 of human origin |
| Modification Notes | Glypican-3 (GPC3) is an integral membrane protein that is mutated in the Simpson-Golabi-Behmel syndrome (SGBS). SGBS is characterized by pre- and post-natal overgrowth and is a recessive X-linked condition. Glypican-3, also designated OCI-5 in rat, is a member of the glypican family of heparan sulfate proteoglycans, which attach to the cell membrane via a glycosyl-phosphatidyl-inositol (GPI) anchor. Expression of glypican-3 is detected in embryonic mesodermal lung, liver and kidney tissues. Glypican-3 is thought to regulate tissue and organ growth through interactions with growth factors such as insulin-like growth factor II (IGF-II) or fibroblast growth factor 2 (FGF-2). Glypican-3 may be downregulated by various means, including promoter hypermethylation or the repression of specific transcription factors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of glypican-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 303-464 mapping near the C-terminus of glypican-3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 303-464 mapping near the C-terminus of glypican-3 of human origin |
| Entrez Gene | 2719 |
|---|
Application
Immunofluorescence staining of methanol-fixed Hep G2 cells showing membrane localization.
Application
Western blot analysis of glypican-3 expression in Hep G2 whole cell lysate.
Application
Western blot analysis of human recombinant glypican-3 fusion protein.| Application Notes | recommended for detection of glypican-3 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
