Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.Application
Blots were probed with p-eIF4E (Ser 209)-R: (A,B,C) and eIF4E (FL-217): preincubated with its cognate phosphorylated peptide (D,E,F).Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of eIF4E expression in SRC-3T3 whole cell lysate.| Product Name | eIF4E Antibody (FL-217) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-217 representing full length eIF4E of human origin |
| Modification Notes | The initiation of protein synthesis in eukaryotic cells is regulated by interactions between protein initiation factors and RNA molecules. The eukaryotic initiation complex eIF4F exists in vitro as a trimeric complex of eIF4G, eIF4E, and eIF4A. Together, the complex allows ribosome binding to mRNA by inducing the unwinding of mRNA secondary structures (1,2). eIF4E binds to the mRNA “cap” during an early step in the initiation of protein synthesis (3,4). eIF4A acts as an ATP-dependent RNA helicase (5,6). eIF4G acts as a bridge between eIF4E, eIF4A, and the eIF3 complex (7,8). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of eIF4E of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-217 representing full length eIF4E of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-217 representing full length eIF4E of human origin |
| Entrez Gene | 1977 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells.
Application
Blots were probed with p-eIF4E (Ser 209)-R: (A,B,C) and eIF4E (FL-217): preincubated with its cognate phosphorylated peptide (D,E,F).
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of eIF4E expression in SRC-3T3 whole cell lysate.| Application Notes | recommended for detection of eIF4E of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
