Application
Western blot analysis of GRB10 expression in HeLa whole cell lysate.| Product Name | GRB10 Antibody (H-130) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-130 mapping at the N-terminus of GRB10 of human origin |
| Modification Notes | Many growth factors function by binding receptors with intrinsic tyrosine kinase activity (1,2). Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine-phosphorylated receptors by a direct interaction between the SH2 domain and specific phospho-tyrosine-containing receptor sequences (3,4). GRB7, a SH2 domain protein, has a single SH2 domain at its C-terminal, a central region with similarity to Ras GAP and a proline-rich N terminus (5,6). A related SH2 domain-containing protein, GRB10, exhibits a high degree of homology with GRB7 (6,7). GRB10 undergoes serine but not tyrosine phosphorylation in response to EGF treatment, but appears to bind to the EGF receptor poorly (7). GRB10 maps to mouse chromosome 11, in close proximity to the EGF receptor (7). Similarly, GRB7 maps to the same mouse chromosome near the EGF receptor-related protein HER2 (6,7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of GRB10 human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-130 mapping at the N-terminus of GRB10 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-130 mapping at the N-terminus of GRB10 of human origin |
| Entrez Gene | 2887 |
|---|
Application
Western blot analysis of GRB10 expression in HeLa whole cell lysate.| Application Notes | recommended for detection of GRB10 human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
