Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of human recombinant megalin.| Product Name | megalin Antibody (H-245) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 4411-4655 mapping at the C-terminus of megalin of human origin |
| Modification Notes | Members of the LDL receptor gene family, including LDLR (low density lipo-protein receptor), LRP (low density lipoprotein related protein), megalin (also designated GP330), VLDLR (very low density lipoprotein receptor) and ApoER2, are characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. Megalin is expressed on the apical membrane domain of epithelial cells, including proximal kidney tubules, intestine and ependymal cells. Proper folding and trafficking of megalin is facilitated by the receptor-associated protein (RAP), a molecular chaperone that can block the uptake of all known ligands for megalin. Specifically, megalin mediates the uptake of apolipoprotein J (apoJ, also designated Clusterin), which is a binding protein for the b-Amyloid peptide, a peptide implicated in Alzheimer’s disease. Megalin is also an antigenic determinant for Heymann nephritis in rats and may be important in the reabsorption of several molecules, including vitamin B12, in the kidney. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of megalin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 4411-4655 mapping at the C-terminus of megalin of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 4411-4655 mapping at the C-terminus of megalin of human origin |
| Entrez Gene | 4036 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human adrenal gland tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of human recombinant megalin.| Application Notes | recommended for detection of megalin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
