Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing membrane and cytoplasmic staining of urothelial cells.Application
Western blot analysis of CAR expression in mouse liver tissue extract.Application
Western blot analysis of CAR expression in non-transfected 293T: (A), mouse CAR transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate cancer showing membrane and cytoplasmic staining of tumor cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse liver showing membrane localization.| Product Name | CAR Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of CAR of human origin |
| Modification Notes | The coxsackie and adenovirus receptor (CAR) mediates viral infection by the binding of various adenoviruses through specific protein interactions. There is a high affinity between the viral knob domain and the extracellular amino- terminal domain (designated D1) of CAR. The D1 domain alone is sufficient for knob binding in transfected cells. Determining the specific interactions between CAR and adenoviruses is imperative in order to further develop gene therapy using viral hosts. CAR is expressed in many human and murine cell types. However, cells that express CAR at low levels are not efficiently infected by adenoviruses. Possible methods of avoiding this problem in certain cell types are by either supplementing CAR or modifying the Ad knob to bind to other receptors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of CAR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of CAR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of CAR of human origin |
| Entrez Gene | 1525 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human urinary bladder tissue showing membrane and cytoplasmic staining of urothelial cells.
Application
Western blot analysis of CAR expression in mouse liver tissue extract.
Application
Western blot analysis of CAR expression in non-transfected 293T: (A), mouse CAR transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate cancer showing membrane and cytoplasmic staining of tumor cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse liver showing membrane localization.| Application Notes | recommended for detection of CAR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
