Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic staining of glandular cells.Application
Western blot analysis of TSHR expression in 293T whole cell lysate.IHC
Effects of RS Extracts and XMU-MP-1 on TSH-R Expression in Thyroid Tissues of Hyperthyroidism Rats by Immunohistochemical Staining (×400)| Product Name | TSHR Antibody (H-155) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Various hormones are secreted from the anterior pituitary during development and growth, including thyroid-stimulating hormone (TSH, also known as thyrotropin), follicle-stimulating hormone (FSH) and leutinizing hormone (LH). TSH, FSH, and LH are heterodimers formed from a common alpha chain and a unique beta chain. TSH is a glycoprotein involved in the control of thyroid structure and metabolism, which stimulates the release of the thyroid hormones (1). TSH is regulated by thyroid hormone (T3) and various retinoid compounds (2). TSH binds to the thyroid-stimulating hormone receptor (TSHR), which is cleaved into two subunits, A and B, and plays a major role in regulating thyroid function (3,4). The third cytoplasmic loop of TSHR has been identified as critical for its role in regulating inositol phosphate and cAMP formation (5). In Graves disease, an autoimmune disorder, TSHR is activated by autoantibodies, which may be stimulated by the cleavage of the A and B subunits (4). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| recommended for detection of TSHR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-155 mapping at the N-terminus of TSHR (thyroid-stimulating hormone receptor) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-155 mapping at the N-terminus of TSHR (thyroid-stimulating hormone receptor) of human origin |
| Entrez Gene | 7253 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human thyroid gland tissue showing cytoplasmic staining of glandular cells.
Application
Western blot analysis of TSHR expression in 293T whole cell lysate.
IHC
Effects of RS Extracts and XMU-MP-1 on TSH-R Expression in Thyroid Tissues of Hyperthyroidism Rats by Immunohistochemical Staining (×400)| Application Notes | recommended for detection of TSHR of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
