Application
Western blot analysis of phosphorylated IκB-ε expression in untreated (A) and TNF-α-treated (B) 293 whole cell lysates.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Product Name | p-IκB-ε Antibody (Ser 22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 22 of IκB-ε of human origin |
| Modification | Phosphoralated |
| Modification Notes | I˚B-ε (I-kappa-B-epsilon), also known as NFKBIE (NFκB inhibitor epsilon) or IKBE, is a 500 amino acid protein that belongs to the NFκB inhibitor family. Localizing to the cytoplasm, I˚B-ε is highly expressed in spleen, testis and lung, with lower levels of expression found in kidney, pancreas, heart, placenta, brain, granulocytes and macrophages. I˚B-ε inhibits c-Rel and NFκB subunits p50, p52 and p65 by forming a complex with them in the cytoplasm, preventing them from activating genes in the nucleus. I˚B-ε undergoes serine phosphorylation, resulting in the protein being marked for destruction via the ubiquitination pathway. Containing six ANK repeats, the gene encoding I˚B-ε maps to human chromosome 6p21.1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 22 phosphorylated IκB-ε of mouse and human origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 22 of I&kappa B-&epsilon of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 22 of IκB-ε of human origin |
| Entrez Gene | 4794 |
|---|
Application
Western blot analysis of phosphorylated IκB-ε expression in untreated (A) and TNF-α-treated (B) 293 whole cell lysates.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing cytoplasmic localization.| Application Notes | recommended for detection of Ser 22 phosphorylated IκB-ε of mouse and human origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
