Application
Antibodies tested include p-FKHRL1 (Ser 253): (A,B,C) and FKHRL1 (m): 293T Lysate: (D,E,F).Application
Antibodies tested include p-FKHRL1 (Ser 253): (A,B,C) and FKHRL1 (H-144): (D,E,F).Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Product Name | p-FKHRL1 Antibody (Ser 253) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to phosphorylated Ser 253 of FKHRL1 of human origin |
| Modification | Phosphoralated |
| Modification Notes | FKHRL1 (for forkhead in rhabdomyosarcoma) is a member of the FKHR subfamily of forkhead transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 at threonine-32 and serine-253. Phosphorylation by Akt1 negatively regulates FKHRL1 by promoting its export from the nucleus. Phosphorylated FKHRL1 associates with 14-3-3 proteins and this complex is retained in the cytoplasm. Growth factor withdrawal stimulates FKHRL1 dephosphorylation and nuclear translocation, leading to FKHR-induced gene-specific transcriptional activation. Within the nucleus, dephospho-rylated FKHRL1 triggers apoptosis by inducing the expression of genes that are critical for cell death. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHC |
| recommended for detection of Ser 253 phosphorylated FKHRL1 of human origin and correspondingly phosphorylated Ser 253 of mouse and rat origin by WB, IP, IF and IHC(P): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to phosphorylated Ser 253 of FKHRL1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to phosphorylated Ser 253 of FKHRL1 of human origin |
| Entrez Gene | 2309 |
|---|
Application
Antibodies tested include p-FKHRL1 (Ser 253): (A,B,C) and FKHRL1 (m): 293T Lysate: (D,E,F).
Application
Antibodies tested include p-FKHRL1 (Ser 253): (A,B,C) and FKHRL1 (H-144): (D,E,F).
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing nuclear localization.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing nuclear staining.| Application Notes | recommended for detection of Ser 253 phosphorylated FKHRL1 of human origin and correspondingly phosphorylated Ser 253 of mouse and rat origin by WB, IP, IF and IHC(P): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
