Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.Application
Immunofluorescence staining of methanol-fixed COLO 320DM cells showing cytoplasmic localization.Application
Western blot analysis of human recombinant FGF-2 tested at 10 ng.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue. Note cytoplasmic staining of epithelial cells.| Product Name | FGF-2 Antibody (147) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of FGF-2 of human origin |
| Modification Notes | Fibroblast growth factor-1 (FGF-1), also designated acidic FGF, and fibroblast growth factor-2 (FGF-2), also designated basic FGF, are members of a family of growth factors that stimulate proliferation of cells of mesenchymal, epithe-lial and neuroectodermal origin. Additional members of the FGF family include the oncogenes FGF-3 (Int2) and FGF-4 (hst/Kaposi), FGF-5, FGF-6, FGF-7 (KGF), FGF-8 (AIGF), FGF-9 (GAF) and FGF-10–FGF-23. Members of the FGF family share 30-55% amino acid sequence identity and similar gene structure, and are capable of transforming cultured cells when overexpressed in transfected cells. Cellular receptors for FGFs are members of a second multigene family including four tyrosine kinases, designated Flg (FGFR-1), Bek (FGFR-L), TKF and FGFR-3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of FGF-2 of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of FGF-2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of FGF-2 of human origin |
| Entrez Gene | 2247 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in tubules.
Application
Immunofluorescence staining of methanol-fixed COLO 320DM cells showing cytoplasmic localization.
Application
Western blot analysis of human recombinant FGF-2 tested at 10 ng.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue. Note cytoplasmic staining of epithelial cells.| Application Notes | recommended for detection of FGF-2 of mouse, rat, human and Xenopus origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
