Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of smooth muscle cells.Application
Western blot analysis of PDGF-A expression in non-transfected: (A) and mouse PDGF-A transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PDGF-A expression in mouse brain tissue extract.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing membrane localization.Application
Western blot analysis of 10 ng of human recombinant PDGF-A showing detection of dimeric form.| Product Name | PDGF-A Antibody (N-30) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of PDGF-A of human origin |
| Modification Notes | PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, PDGF AB, and PDGF BB. The three isoforms bind with different affinities to two receptor types, å and ∫, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers; the A subunit of PDGF binds only to å receptors with high affinity, whereas the B subunit can bind to both å and ∫ receptors. Evidence suggests that PDGF may function as a neurotrophic factor. The fact that receptors for DGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PDGF-A of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PDGF-A of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PDGF-A of human origin |
| Entrez Gene | 5154 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of smooth muscle cells.
Application
Western blot analysis of PDGF-A expression in non-transfected: (A) and mouse PDGF-A transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PDGF-A expression in mouse brain tissue extract.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing membrane localization.
Application
Western blot analysis of 10 ng of human recombinant PDGF-A showing detection of dimeric form.| Application Notes | recommended for detection of PDGF-A of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
