Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.Application
Western blot analysis of VEGF expression in non-transfected: (A) and human VEGF transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human soft tissue showing cytoplasmic staining of chondrocytes, endothelial cells, and perineural cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of human recombinant VEGF.Application
Antibodies tested include VEGF (147): (A) and VEGF (A-20): (B).Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human kidney showing diffuse cytoplasmic staining of kidney tubules (A). Immunofluorescence staining of PMA-treated, methanol-fixed SK-BR-3 cells, showing cytoplasmic fluorescein immunostaining and nuclear DAPI counterstain (B).Application
Western blot analysis of reduced (A) and unreduced (B) purified VEGF protein (20 ng).| Product Name | VEGF Antibody (A-20) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of VEGF-A of human origin |
| Conjugation | Agarose |
| Modification | Acetylated |
| Modification Notes | The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factors (FGFs), platelet derived growth factor (PDGF) and vascular endothelial growth factor (VEGF). VEGF is a dimeric glycoprotein with structural homology to PDGF. Several variants of VEGF have been described that arise by alternative mRNA splicing. It has been speculated that VEGF may function as a tumor angiogenesis factor in vivo because the expression pattern of VEGF is consistent with a role in embryonic angiogenesis. VEGF mRNA is formed in some primary tumors, VEGF is produced by tumor cell lines in vitro and VEGF mitogenic activity appears to be restricted to endothelial cells. A member of the PDGF receptor family, Flt, has been identified as a high-affinity receptor for VEGF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHCIPWB |
| WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | bovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of VEGF-A of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of VEGF-A of human origin |
| Entrez Gene | 7422 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cervix tissue showing cytoplasmic staining of squamous epithelial cells.
Application
Western blot analysis of VEGF expression in non-transfected: (A) and human VEGF transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human soft tissue showing cytoplasmic staining of chondrocytes, endothelial cells, and perineural cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of human recombinant VEGF.
Application
Antibodies tested include VEGF (147): (A) and VEGF (A-20): (B).
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human kidney showing diffuse cytoplasmic staining of kidney tubules (A). Immunofluorescence staining of PMA-treated, methanol-fixed SK-BR-3 cells, showing cytoplasmic fluorescein immunostaining and nuclear DAPI counterstain (B).
Application
Western blot analysis of reduced (A) and unreduced (B) purified VEGF protein (20 ng).| Application Notes | WB:1:100~1:500 IP:1:20~1:100 IF:1:20~1:100 IHC:1:10~1:50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
