Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing cytoplasmic staining of cells in fallopian tube.Application
Western blot analysis of PDGF-A expression in mouse brain tissue extract.Application
Immunofluorescence staining of normal mouse liver frozen section showing extracellular matrix staining.Application
Western blot analysis of human recombinant PDGF-A under non-reducing conditions.| Product Name | PDGF-A Antibody (H-77) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 135-211 mapping at the C-terminus of PDGF-A of human origin |
| Modification Notes | PDGF is a mitogen for mesenchyme- and glia-derived cells. It consists of two disulfide-bonded polypeptide chains, A and B, and occurs as three isoforms, PDGF AA, PDGF AB, and PDGF BB. The three isoforms bind with different affinities to two receptor types, å and ∫, which are structurally related and endowed with protein-tyrosine kinase domains. Ligand binding induces activation of the receptor kinases by formation of receptor dimers; the A subunit of PDGF binds only to å receptors with high affinity, whereas the B subunit can bind to both å and ∫ receptors. Evidence suggests that PDGF may function as a neurotrophic factor. The fact that receptors for DGF-A are expressed in oligodendrocyte progenitor cells whereas receptors for PDGF-B are expressed on neurons suggests that the different isoforms of PDGF may regulate growth and differentiation of different cell types in the developing central nervous system by paracrine and autocrine routes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of PDGF-A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 135-211 mapping at the C-terminus of PDGF-A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 135-211 mapping at the C-terminus of PDGF-A of human origin |
| Entrez Gene | 5154 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human ovary tissue showing cytoplasmic staining of cells in fallopian tube.
Application
Western blot analysis of PDGF-A expression in mouse brain tissue extract.
Application
Immunofluorescence staining of normal mouse liver frozen section showing extracellular matrix staining.
Application
Western blot analysis of human recombinant PDGF-A under non-reducing conditions.| Application Notes | recommended for detection of PDGF-A of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
