Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of Bmx expression in non-transfected: (A) and human Bmx transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human smooth muscle tissue showing cytoplasmic staining of endothelial cells in low (A) and high (B) resolution. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Bmx expression in non-transfected: (A) and human Bmx transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Bmx expression in non-transfected: (A) and human Bmx transfected: (B) 293T whole cell lysates.| Product Name | Bmx Antibody (H-220) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 81-300 mapping near the N-terminus of Bmx of human origin |
| Modification Notes | The Tec family of non-receptor tyrosine kinases is composed of six proteins, designated Tec, Emt (also known as Itk or Tsk), Btk (previously known as Atk, BPK or Emb), Bmx, Txk (also known as Rlk) and Dsrc28C. All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a Pleckstrin homology (PH) and a Tec homology (TH) domain in their amino-termini. Four alternatively spliced forms of Tec are found to be expressed broadly in cells of hematopoietic lineage and in hepatocytes. The Emt gene product associates with CD28 and becomes activated subsequent to CD28 ligation. Btk is necessary for proper B cell development, and mutations in the gene encoding Btk have been associated with families suffering from X-linked agammaglobulinemia, also referred to as Bruton’s disease. The Bmx protein shares a high degree of homology with Btk and seems to be expressed at highest levels in the heart. Txk expression is T cell-specific, while expression of the Drosophila Tec homolog, Dsrc28C, is developmentally regulated. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Bmx of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 81-300 mapping near the N-terminus of Bmx of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 81-300 mapping near the N-terminus of Bmx of human origin |
| Entrez Gene | 660 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of Bmx expression in non-transfected: (A) and human Bmx transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human smooth muscle tissue showing cytoplasmic staining of endothelial cells in low (A) and high (B) resolution. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Bmx expression in non-transfected: (A) and human Bmx transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Bmx expression in non-transfected: (A) and human Bmx transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Bmx of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
