Application
Western blot analysis of SH-PTP1 expression in non-transfected 293T: (A), mouse SH-PTP1 transfected 293T: (B) and HL-60 (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.Application
Western blot analysis of SH-PTP1 expression in HEL 92.1.7 (A) and MEG-01 (B) whole cell lysates.Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of SH-PTP1 expression in HL-60 (A) and Jurkat (B) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HL-60 cells showing cytoplasmic staining.Application
Western blot analysis of SH-PTP1 expression in U-937 (A), HL-60 (B) and Jurkat (C) whole cell lysates.| Product Name | SH-PTP1 Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The steady state of protein tyrosyl phosphorylation in cells is regulated by the opposing action of tyrosine kinases and protein tyrosine phosphatases (PTPs). Several groups have independently identified a non-transmembrane PTP, designated SH-PTP1 (also known as PTP1C, HCP and SHP), which is primarily expressed in hematopoietic cells and characterized by the presence of two SH2 domains N-terminal to the PTP domain. SH2 domains generally mediate the association of regulatory molecules with specific phosphotyrosine-containing sites on autophosphorylated receptors, thereby controlling the initial interaction of receptors with these substrates. A second and much more widely expressed PTP with SH2 domains, SH-PTP2 (also designated PTP1D and Syp), has been identified. Strong sequence similarity between SH-PTP2 and the Drosophila gene corkscrew (CSW) and their similar patterns of expression suggest that SH-PTP2 is the human corkscrew homolog. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,FC,ELISA |
| recommended for detection of SH-PTP1 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine and porcine | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of SH-PTP1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of SH-PTP1 of human origin |
| Entrez Gene | 5777 |
|---|
Application
Western blot analysis of SH-PTP1 expression in non-transfected 293T: (A), mouse SH-PTP1 transfected 293T: (B) and HL-60 (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic staining.
Application
Western blot analysis of SH-PTP1 expression in HEL 92.1.7 (A) and MEG-01 (B) whole cell lysates.
Application
Intracellular FCM analysis of fixed and permeabilized Jurkat cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of SH-PTP1 expression in HL-60 (A) and Jurkat (B) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HL-60 cells showing cytoplasmic staining.
Application
Western blot analysis of SH-PTP1 expression in U-937 (A), HL-60 (B) and Jurkat (C) whole cell lysates.| Application Notes | recommended for detection of SH-PTP1 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
