Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and membrane localization.Application
Western blot analysis of PKA IIα regulatory subunit expression in Mv 1 Lu (A) and MDCK (B) whole cell lysates.| Product Name | PKA IIα reg Antibody (M-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified, designated Cå, C∫ and C©, that each represent specific gene products. Cå and C∫ are closely related (93% amino acid sequence similarity), whereas C© displays 83% and 79% similarity to Cå and C∫, respectively. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcription factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,ELISA |
| recommended for detection of the PKA II&alpha and, to a lesser extent, PKA II&beta regulatory subunit of mouse, rat, canine and mink and, to a lesser extent, human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PKA II&alpha reg of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PKA IIα reg of mouse origin |
| Entrez Gene | 5576 |
|---|
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic and membrane localization.
Application
Western blot analysis of PKA IIα regulatory subunit expression in Mv 1 Lu (A) and MDCK (B) whole cell lysates.| Application Notes | recommended for detection of the PKA II&alpha and, to a lesser extent, PKA II&beta regulatory subunit of mouse, rat, canine and mink and, to a lesser extent, human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
