Application
Western blot analysis of Hck expression in non-transfected 293T: (A), mouse Hck transfected 293T: (B) and HL-60 (C) whole cell lysates.| Product Name | Hck Antibody (H-85) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-85 of Hck of human origin |
| Modification Notes | Src is the human homolog of the v-src gene of the Rous sarcoma virus, also called avian sarcoma virus or ASV. Src was the first proto-oncogenic non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility and adhesion. Src family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Src family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. Different subcellular localizations of Src family kinases may be important for the regulation of specific cellular processes such as mitogenesis, cytoskeletal organization and membrane trafficking. The human hemopoietic cell kinase (Hck) gene maps to chromosome 20q11-q12 and encodes a 505 amino acid protein. The Hck protein is expressed in hematopoietic cells, and is particularly abundant in granulocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Hck p59 and Hck p60 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-85 of Hck of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-85 of Hck of human origin |
| Entrez Gene | 3055 |
|---|
Application
Western blot analysis of Hck expression in non-transfected 293T: (A), mouse Hck transfected 293T: (B) and HL-60 (C) whole cell lysates.| Application Notes | recommended for detection of Hck p59 and Hck p60 of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
