Application
Western blot analysis of IARS2 expression in non-transfected 293T: (A), human IARS2 transfected 293T: (B), HEK293 (C), K-562 (D) and RAW 264.7 (E) whole cell lysates.Application
Western blot analysis of IARS2 expression in non-transfected 293T: (A), human IARS2 transfected 293T: (B), HEK293 (C), K-562 (D) and RAW 264.7 (E) whole cell lysates.Application
Western blot analysis of IARS2 expression in K-562 whole cell lysate.Application
Western blot analysis of IARS2 expression in 293 whole cell lysate.| Product Name | IARS2 Antibody (A-22) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of IARS2 of human origin by WB, IP and ELISA |
| Modification Notes | The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Proteins belonging to this family function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. IARS2 (Isoleucyl-tRNA synthetase), also known as IleRS (Isoleucine-tRNA ligase), is a 1012 amino acid protein that is localized within the mitochondrial matrix. Specifically belonging to the class I mitochondrial aminoacyl-tRNA synthetase family, IARS2 contains a characteristic Rossman fold, which is found in proteins that typically bind nucleotides. The gene encoding IARS2 maps to human chromosome one, which is the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPELISA |
| WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of IARS2 of human origin by WB IP and ELISA |
|---|---|
| Tissue Specificity | recommended for detection of IARS2 of human origin by WB, IP and ELISA |
| Entrez Gene | 55699 |
|---|
Application
Western blot analysis of IARS2 expression in non-transfected 293T: (A), human IARS2 transfected 293T: (B), HEK293 (C), K-562 (D) and RAW 264.7 (E) whole cell lysates.
Application
Western blot analysis of IARS2 expression in non-transfected 293T: (A), human IARS2 transfected 293T: (B), HEK293 (C), K-562 (D) and RAW 264.7 (E) whole cell lysates.
Application
Western blot analysis of IARS2 expression in K-562 whole cell lysate.
Application
Western blot analysis of IARS2 expression in 293 whole cell lysate.| Application Notes | WB:1:500~1:2000 IP:1:100~1:500 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
