Application
Western blot analysis of TGIF expression in non-transfected: (A) and mouse TGIF transfected: (B) 293T whole cell lysates.Application
Western blot analysis of TGIF expression in JAR whole cell lysate.Application
Western blot analysis of TGIF expression in non-transfected: (A) and human TGIF transfected: (B) 293 whole cell lysates.Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.Application
Western blot analysis of TGIF expression in SW480 nuclear extract.| Product Name | TGIF Antibody (H-172) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 100-272 mapping at the C-terminus of TGIF (5'-TG-3' interacting factor) of human origin |
| Modification Notes | TGIF (5’-TG-3’ interacting factor) was originally identified as a homeo-domain protein that binds to a retinoid X receptor (RXR) responsive element, thereby inhibiting the binding of RXR to this site and repressing RXR-dependent transcriptional activation. TGIF is a member of the TALE (three amino acid loop extension) family of homeodomain-containing proteins. TGIF also binds to Smad2, to repress Smad2-Smad4-mediated transcription. Smad2, after phosphorylation mediated by TGF∫ receptor, forms a complex with Smad4 and enters the nucleus to regulate transcription. The Smad2-Smad4 complex can interact with coactivators to form a transcriptional activation complex. Alternatively, the Smad2-Smad4 complex can interact with TGIF and HDACs to form a transcriptional repressor complex. Upon interaction with Smad2, TGIF is recruited to TGF∫-responsive genes, where it acts to repress TGF∫-induced transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of TGIF of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 100-272 mapping at the C-terminus of TGIF (5'-TG-3' interacting factor) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 100-272 mapping at the C-terminus of TGIF (5'-TG-3' interacting factor) of human origin |
| Entrez Gene | 7050 |
|---|
Application
Western blot analysis of TGIF expression in non-transfected: (A) and mouse TGIF transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of TGIF expression in JAR whole cell lysate.
Application
Western blot analysis of TGIF expression in non-transfected: (A) and human TGIF transfected: (B) 293 whole cell lysates.
Application
Immunofluorescence staining of normal mouse liver frozen section showing nuclear staining.
Application
Western blot analysis of TGIF expression in SW480 nuclear extract.| Application Notes | recommended for detection of TGIF of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
