Application
Western blot analysis of SEC14L2 expression in non-transfected: (A) and mouse SEC14L2 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of SEC14L2 expression in rat liver tissue extract.Application
Western blot analysis of SEC14L2 expression in non-transfected: (A) and mouse SEC14L2 transfected: (B) 293T whole cell lysates and mouse liver tissue extract (C).Application
Western blot analysis of SEC14L2 expression in mouse liver tissue extract.| Product Name | SEC14L2 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of SEC14L2 of human origin |
| Modification Notes | The monomeric, SEC14L2 (SEC14-like protein 2), also known as supernatant protein factor (SPF), α-tocopherol-associated protein or squalene transfer protein, functions as a carrier protein transferring tocopherols, as a transcriptional activator via its interaction with α-tocopherol and as a stimulator of conversion of microsomal squalene-2,3-oxide into lanosterol in cholesterol biosynthesis. High levels of SEC14L2 are expressed in liver, brain, intestine and prostate. Subcellular localization of SEC14L2 is cytoplasmic, but in the presence of α-tocopherol, SEC14L2 localizes in the nucleus. Activity of SEC14L2 depends on posttranslational modifications, specifically phosphorylation by PKA and PKC. SEC14L3 and SEC14L4 both contain one CRAL-TRIO domain and one GOLD domain and may be involved in the transport of hydrophobic ligands. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SEC14L2 of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with SEC14L3 and SEC14L4 also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of SEC14L2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of SEC14L2 of human origin |
| Entrez Gene | 23541 |
|---|
Application
Western blot analysis of SEC14L2 expression in non-transfected: (A) and mouse SEC14L2 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of SEC14L2 expression in rat liver tissue extract.
Application
Western blot analysis of SEC14L2 expression in non-transfected: (A) and mouse SEC14L2 transfected: (B) 293T whole cell lysates and mouse liver tissue extract (C).
Application
Western blot analysis of SEC14L2 expression in mouse liver tissue extract.| Application Notes | recommended for detection of SEC14L2 of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with SEC14L3 and SEC14L4 also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
