Application
Western blot analysis of Mi2 expression in 293T (A) and K-562 (B) whole cell lysates.Application
Western blot analysis of Mi2 expression in K-562 nuclear extract.| Product Name | Mi2 Antibody (H-242) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1671–1912 of Mi2-β of human origin |
| Modification Notes | In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Chromatin structure alteration may be brought about by the action of ATP-dependent multiprotein complexes. One such complex is the mSin3 corepressor complex, which contains mSin3, the histone deacetylases HDAC1 and HDAC2, the associated proteins SAP 30 and SAP 18, and the autoantigens Mi2-a and Mi2-b. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAChIP |
| recommended for detection of Mi2-α and Mi2-β of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1671–1912 of Mi2-&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1671–1912 of Mi2-β of human origin |
| Entrez Gene | 1108 |
|---|
Application
Western blot analysis of Mi2 expression in 293T (A) and K-562 (B) whole cell lysates.
Application
Western blot analysis of Mi2 expression in K-562 nuclear extract.| Application Notes | recommended for detection of Mi2-α and Mi2-β of human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
