Application
Western blot analysis of INSM1 expression in truncated human recombinant INSM1 fusion protein.Application
Western blot analysis of INSM1 expression in non-transfected 293T: (A), mouse INSM1 transfected 293T: (B) and TT (C) whole cell lysates.| Product Name | INSM1 Antibody (H-45) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 81-125 mapping near the N-terminus of INSM1 of human origin |
| Modification Notes | INSM1 (Insulinoma-associated protein 1), also known as zinc finger protein IA-1, is a developmentally regulated zinc finger transcription factor. It localizes to the nucleus and is expressed in embryonic tissues undergoing neuroendocrine differentiation. INSM1 is not expressed in normal adult tissues but it can be found highly expressed in neuroendocrine tumors. INSM1 contains 5 Cys(2)-His(2)-type zinc finger DNA binding domains and a prohormone domain. INSM1 acts as a transcriptional repressor of the Neuro D promoter and recruits cyclin D1 as a co-repressor. It plays an important role in neuroendocrine development and is required for normal differentiation of pancreatic endocrine cells. Inhibition of INSM1 results in decreased formation of glucagon and insulin positive cells. The gene encoding INSM1 is directly regulated by Neurogenin 3 which binds chromatin in the INSM1 promoter region and induces transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of insulinoma-associated 1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 81-125 mapping near the N-terminus of INSM1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 81-125 mapping near the N-terminus of INSM1 of human origin |
| Entrez Gene | 3642 |
|---|
Application
Western blot analysis of INSM1 expression in truncated human recombinant INSM1 fusion protein.
Application
Western blot analysis of INSM1 expression in non-transfected 293T: (A), mouse INSM1 transfected 293T: (B) and TT (C) whole cell lysates.| Application Notes | recommended for detection of insulinoma-associated 1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
