Application
Western blot analysis of ENX-1 expression in non-transfected: (A) and mouse ENX-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ENX-1 expression in non-transfected: (A) and mouse ENX-1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ENX-1 expression in non-transfected: (A) and human ENX-1 transfected: (B) 293 whole cell lysates.Application
Western blot analysis of ENX-1 expression in non-transfected: (A) and human ENX-1 transfected: (B) CHO whole cell lysates.| Product Name | ENX-1 Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In Drosophila, the Polycomb (PcG) gene family encodes chromatin proteins that are required for the repression of homeotic loci in embryonic development. PcG proteins work in conjunction with the trithorax-group (trxG), which activate homeobox gene expression during embryonic development. ENX-1, a mammalian homolog of the Drosophila gene enhancer of zeste, is a PcG protein that is ubiquitously expressed during early embryogenesis and becomes restricted to the central and peripheral nervous systems and sites of fetal hematopoiesis during later development. In the adult, ENX-1 is restricted to specific sites, including spleen, testis and placenta. The gene encoding human ENX-1 transcribes a 746 amino acid polypeptide which contains a trithorax-like domain and a DNA-binding motif. ENX-1 interacts with the proto-oncogene product Vav and is thought to be involved in the proliferation of normal and malignant hematopoietic cells. By altering the regulation of target genes, ENX-1 may also contribute to certain phenotypes of Down syndrome. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,ELISA,GS,ChIP |
| recommended for detection of ENX-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 31-110 mapping at the N-terminus of ENX-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 31-110 mapping at the N-terminus of ENX-1 of human origin |
| Entrez Gene | 2146 |
|---|
Application
Western blot analysis of ENX-1 expression in non-transfected: (A) and mouse ENX-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ENX-1 expression in non-transfected: (A) and mouse ENX-1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ENX-1 expression in non-transfected: (A) and human ENX-1 transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of ENX-1 expression in non-transfected: (A) and human ENX-1 transfected: (B) CHO whole cell lysates.| Application Notes | recommended for detection of ENX-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
