Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization (A) and nuclear localization after UV exposure (B).Application
Western blot analysis of DDB2 expression in untreated HeLa (A) and UV-treated HeLa (B) nuclear extracts.| Product Name | DDB2 Antibody (H-127) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 301-427 mapping at the C-terminus of DDB2 of human origin |
| Modification Notes | Damaged DNA binding protein (DDB) is a heterodimer composed of two subunits, p127 and p48, which are designated DDB1 and DDB2, respectively (1-4). The DDB heterodimer is involved in repairing DNA damaged by ultraviolet light (1,2). Specifically, DDB, also designated UV-damaged DNA binding protein (UV-DDB), xeroderma pigmentosum group E binding factor (XPE-BF) and hepatitis B virus X-associated protein 1 (XAP-1), binds to damaged cyclobutane pyrimidine dimers (CPDs) (5,6). Mutations in the DDB2 gene are implicated as causes of xeroderma pigmentosum group E, an autosomal recessive disease in which patients are defective in nucleotide excision DNA repair (1,2,7,8). XPE is characterized by hypersensitivity of the skin to sunlight with a high frequency of skin cancer as well as neurologic abnormalities (7,8). The hepatitis B virus (HBV) X protein interacts with DDB1, which may mediate HBx transactivation (9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DDB2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 301-427 mapping at the C-terminus of DDB2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 301-427 mapping at the C-terminus of DDB2 of human origin |
| Entrez Gene | 1643 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization (A) and nuclear localization after UV exposure (B).
Application
Western blot analysis of DDB2 expression in untreated HeLa (A) and UV-treated HeLa (B) nuclear extracts.| Application Notes | recommended for detection of DDB2 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
