Application
ChIP analysis of TFIIE-α recruitment to genomic amplicons. Two (1,2) different human genomic TBP and three control amplicons (3-5) were analyzed by quantitative PCR (primer sequences available in on-line supplemental data). Data generated in collaboration with Drs. N. Trinklein and R. Myers, Stanford University (ENCODE Project).Application
Western blot analysis of TFIIE-α expression in non-transfected control (A) and TFIIE-α siRNA transfected (B) HeLa cells. Blot probed with TFIIE-α (C-17): . GAPDH (FL-335): used as specificity and loading control.Application
Western blot analysis of TFIIE-α expression in NIH/3T3 nuclear extract.| Product Name | TFIIE-α Antibody (C-17) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of TFIIE-α of human origin |
| Modification Notes | In eukaryotic systems, initiation of transcription from protein-coding genes is a complex process requiring RNA polymerase II and broad families of auxiliary transcription factors. Such factors can be divided into two major functional classes: the basal factors that are required for transcription of all Pol II genes, including TFIIA, TFIIB, TFIID, TFIIE, TFIIF and TFIIH; and sequence-specific factors that regulate gene expression. The basal transcription factors and Pol II form a specific multiprotein complex near the transcription start site by interacting with core promotor elements such as the TATA box generally located 25-30 base pairs upstream of the transcription start site. Human TFIIE consists of two subunits, a and b. The structure of TFIIE appears to be a heterotetramer (å2∫2); both subunits are required for optimal basal-level transcription. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of TFIIE-α p57 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of TFIIE-&alpha of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of TFIIE-α of human origin |
| Entrez Gene | 2960 |
|---|
Application
ChIP analysis of TFIIE-α recruitment to genomic amplicons. Two (1,2) different human genomic TBP and three control amplicons (3-5) were analyzed by quantitative PCR (primer sequences available in on-line supplemental data). Data generated in collaboration with Drs. N. Trinklein and R. Myers, Stanford University (ENCODE Project).
Application
Western blot analysis of TFIIE-α expression in non-transfected control (A) and TFIIE-α siRNA transfected (B) HeLa cells. Blot probed with TFIIE-α (C-17): . GAPDH (FL-335): used as specificity and loading control.
Application
Western blot analysis of TFIIE-α expression in NIH/3T3 nuclear extract.| Application Notes | recommended for detection of TFIIE-α p57 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
