Application
Western blot analysis of EFP expression in HeLa (A), C32 (B) and MCF7 (C) nuclear extracts.Application
Western blot analysis of EFP expression in non-transfected 293T: (A), human EFP transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of EFP expression in non-transfected: (A) and mouse EFP transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Western blot analysis of EFP expression in non-transfected: (A) and human EFP transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.Application
Western blot analysis of EFP expression in HeLa (A) and C32 (B) nuclear extracts.| Product Name | EFP Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 331-630 mapping at the C-terminus of EFP of human origin |
| Modification Notes | EFP (estrogen-responsive finger protein) is a transcription factor, the content of which is regulated by estrogen. It has been identified as a member of the RING finger family, a family of proteins containing a Zn2+ binding domain designated the C3HC4 or RING finger. EFP also contains two B box domains and a coiled-coil region (a transactivation domain), which are characteristic of a subgroup of the RING finger family. Estrogen regulates the growth, differentiation and function of target cells in a variety of tissues; however, few genes have been shown to be directly regulated by estrogen. It has been speculated that EFP may mediate estrogen activity in a signaling cascade in which estrogen-ER binding to the estrogen responsive element (ERE) downstream of the EFP gene upregulates EFP gene expression. The EFP gene product may then activate transcription of secondary estrogen responsive genes. Additional studies indicate that the EFP promoter may be regulated by multiple elements and their interacting factors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of EFP of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 331-630 mapping at the C-terminus of EFP of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 331-630 mapping at the C-terminus of EFP of human origin |
| Entrez Gene | 7706 |
|---|
Application
Western blot analysis of EFP expression in HeLa (A), C32 (B) and MCF7 (C) nuclear extracts.
Application
Western blot analysis of EFP expression in non-transfected 293T: (A), human EFP transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of EFP expression in non-transfected: (A) and mouse EFP transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Western blot analysis of EFP expression in non-transfected: (A) and human EFP transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.
Application
Western blot analysis of EFP expression in HeLa (A) and C32 (B) nuclear extracts.| Application Notes | recommended for detection of EFP of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
