Application
Western blot analysis of NF-E2 expression in non-transfected control (A) and NF-E2 siRNA transfected (B) HeLa cells. Blot probed with NF-E2 (C-19): . GAPDH (FL-335): used as specificity and loading control.Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with NF-E2 (C-19), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.Application
Western blot analysis of NF-E2 expression in K-562 nuclear extract.| Product Name | NF-E2 Antibody (C-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of NF-E2 of mouse origin |
| Modification Notes | The nuclear DNA binding protein NF-E2 regulates expression of globulin genes in developing erythroid cells through interaction with upstream AP-1-like recognition sites. More specifically, NF-E2 recognizes a site containing an intact AP-1 binding motif, preceded by a G residue two base pairs upstream. NF-E2 is an obligate heterodimer composed of NF-E2 p45 and NF-E2 p18. NF-E2 p18, also known as NF-E2U or MAFK, is a ubiquitously expressed component that is related to the v-Maf oncogene. It contains a basic-leucine zipper domain that functions in DNA binding and dimerization. In addition, NF-E2 p18 may play a role in erythroid differentiation. The major component of NF-E2 is a polypeptide, designated NF-E2 p45, that belongs to the basic region-leucine zipper family of transcription factors. This subunit of NF-E2 is specifically expressed at low level in hematopoietic progenitor cells and differentiated cells of the erythroid, megakaryocyte and mast cell lineages. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISAGSChIP |
| recommended for detection of NF-E2 p45 of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739 also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of NF-E2 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of NF-E2 of mouse origin |
| Entrez Gene | 4778 |
|---|
Application
Western blot analysis of NF-E2 expression in non-transfected control (A) and NF-E2 siRNA transfected (B) HeLa cells. Blot probed with NF-E2 (C-19): . GAPDH (FL-335): used as specificity and loading control.
Application
Indirect, intracellular FCM analysis of fixed and permeabilized K-562 cells stained with NF-E2 (C-19), followed by PE-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear staining.
Application
Western blot analysis of NF-E2 expression in K-562 nuclear extract.| Application Notes | recommended for detection of NF-E2 p45 of mouse, rat and human origin by WB, IP, IF, ELISA and indirect FCM using PE-conjugated goat anti-rabbit IgG: sc-3739 also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
