Application
Western blot analysis of PEBP2β expression in non-transfected: (A) and human PEBP2β transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PEBP2β expression in non-transfected: (A) and mouse PEBP2β transfected: (B) 293T whole cell lysates.Application
Western blot analysis of PEBP2β expression in non-transfected: (A) and human PEBP2β transfected: (B) whole cell lysates.Application
Western blot analysis of PEBP2β expression in HeLa whole cell lysate.Application
Western blot analysis of PEBP2β expression in HEK293 whole cell lysate.Application
Western blot analysis of PEBP2β expression in MEG-01 whole cell lysate.| Product Name | PEBP2β Antibody (FL-182) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-182 representing full length PEBP2β of human origin |
| Modification Notes | The transcription factor Polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (Osteoblast-specific transcription factor), CBFA1 (Core Binding Factor) and AML3 (Acute myeloid leukemia), is composed of two subunits, a and b, which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2a subunits, PEBP2aA, PEBP2aB and PEBP2aC, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, Runt. This region is involved in DNA binding and heterodimerization with the regulatory b subunit, which facilitates DNA binding of the a subunit. Both subunits are required for in vivo function; the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2b is the target of chromosomal inversion 16 (p13;q22) with the smooth muscle myosin heavy chain, producing a chimeric gene, PEBP2b/CBFB-SMMHC, that is associated with human acute myeloid leukemia. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of PEBP2β of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-182 representing full length PEBP2&beta of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-182 representing full length PEBP2β of human origin |
| Entrez Gene | 865 |
|---|
Application
Western blot analysis of PEBP2β expression in non-transfected: (A) and human PEBP2β transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PEBP2β expression in non-transfected: (A) and mouse PEBP2β transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of PEBP2β expression in non-transfected: (A) and human PEBP2β transfected: (B) whole cell lysates.
Application
Western blot analysis of PEBP2β expression in HeLa whole cell lysate.
Application
Western blot analysis of PEBP2β expression in HEK293 whole cell lysate.
Application
Western blot analysis of PEBP2β expression in MEG-01 whole cell lysate.| Application Notes | recommended for detection of PEBP2β of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
