Application
Western blot analysis of PML expression in non-transfected: (A) and truncated mouse PML transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human renal cancer tissue showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of human recombinant PML fusion protein.Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.| Product Name | PML Antibody (H-238) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 157-394 mapping to an internal region of PML of human origin |
| Modification Notes | The PML protein is a zinc finger transcription factor expressed as three major transcription products due to alternative splicing. The gene encoding human PML maps to chromosome 15q22. The t(15;17) (q22;q11.2-q12) chromosomal translocation of the retinoic acid receptor å (RARå) gene occurs in virtually all cases of acute promyelocytic leukemia and results in the expression of a PML/RARa chimeric protein. Myeloid precursor cells expressing the PML/RARå chimera fail to differentiate and exhibit an increased growth rate consequent to diminished apoptosis. PML/RARå transforms myeloid precursors by recruiting the nuclear co-repressor (N-CoR)-histone deacetylase complex that is essential to retinoic acid-dependent myeloid differentiation. PML/RARå also recruits DNA methyltransferases in order to induce gene hypermethylation and silencing, which ultimately facilitates leukemogenesis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of all isoforms of PML of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 157-394 mapping to an internal region of PML of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 157-394 mapping to an internal region of PML of human origin |
| Entrez Gene | 5371 |
|---|
Application
Western blot analysis of PML expression in non-transfected: (A) and truncated mouse PML transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human renal cancer tissue showing nuclear staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of human recombinant PML fusion protein.
Application
Immunofluorescence staining of methanol-fixed K-562 cells showing nuclear localization.| Application Notes | recommended for detection of all isoforms of PML of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
