Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of AP-2α expression in non-transfected: (A) and mouse AP-2α transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AP-2α expression in non-transfected: (A) and mouse AP-2α transfected: (B) 293T whole cell lysates.Application
Western blot analysis of AP-2α expression in non-transfected: (A) and human AP-2α transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.Application
Western blot analysis of AP-2α expression in HeLa nuclear extract.| Product Name | AP-2α Antibody (H-79) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 130-209 mapping within an internal region of AP-2α of human origin |
| Modification Notes | AP-2 transcription factor family members include AP-2å, AP-2∫ and AP-2©, which specifically bind to the DNA consensus sequence CCCCAGGC and initiate transcription of selected genes. AP-2, also known as ERF-1, plays a role in regulating estrogen receptor expression. AP-2∫, a splice variant of AP-2å, inhibits AP-2 activity. Besides subscribing to the AP-2 complex, AP-2å, AP-2∫ and AP-2© proteins compose the OB2-1 transcription factor complex. OB2-1 specifically upregulates expression of the proto-oncogene c-ErbB-2, which is overexpressed in 25-30% of breast cancers. The gene encoding AP-2å maps to human chromosome 6p24. AP-2å may play an important role in the development of ectodermal-derived tissues. Deleterious mutations involving the AP-2å gene are linked to microphthalmia, corneal clouding and other anterior eye chamber defects. The ubiquitously expressed AP-4 transcription factor specifically binds to the DNA consensus sequence 5’-CAGCTG-3’. AP-4 interacts with promoters for immunoglobulin-˚ gene families and simian virus 40. AP-4 may enhance the transcription of the human Huntington’s disease gene. AP-4 is a helix-loop-helix protein that contains two distinctive leucine repeat elements. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of AP-2α of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 130-209 mapping within an internal region of AP-2&alpha of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 130-209 mapping within an internal region of AP-2α of human origin |
| Entrez Gene | 7020 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of AP-2α expression in non-transfected: (A) and mouse AP-2α transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AP-2α expression in non-transfected: (A) and mouse AP-2α transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of AP-2α expression in non-transfected: (A) and human AP-2α transfected: (B) 293T whole cell lysates and HeLa nuclear extract (C).
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.
Application
Western blot analysis of AP-2α expression in HeLa nuclear extract.| Application Notes | recommended for detection of AP-2α of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
