Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of Purkinje cells.Application
Western blot analysis of Rent1 expression in SK-N-SH whole cell lysate.Application
Western blot analysis of Rent1 expression in Sol8 (A), SK-N-SH (B) and L6 (C) whole cell lysates.| Product Name | Rent1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 71-370 mapping near the N-terminus of Rent1 of human origin |
| Modification Notes | In eukaryotes, it is essential to have the ability to detect and degrade transcripts that lack full coding potential. Nonsense-mediated RNA decay (NMD) protects the organism by avoiding the translation of truncated peptides with dominant negative or deleterious gain-of-function potential. Rent1, a mammalian ortholog of Upflp, is essential for embryonic viability (1–3). Rent1 (also designated regulator of nonsense transcripts and HUpf1) contains an N-terminal zinc finger-like domain, NTPase domains and a region comprised of domains that define Rent1 as a superfamily group I helicase. Rent1 protein has nucleic-acid-dependent ATPase activity and 5' to 3' helicase activity. In addition, Rent1 is an RNA-binding protein whose activity is modulated by ATP (4,5) and directly interacts with Rent2, which is a mammalian homolog of Upf2p (2). Two mammalian orthologs to Upf3p, Rent3a and Rent3b, are encoded by two separate genes (6). Rent3b (also known as Rent3X) is encoded by a X-linked gene and localizes primarily to the nucleus, while Rent 1 and Rent 2 localize primarily in the cytoplasm (6). Specific Rent3 protein interactions with Y14 and spliced mRNA suggest Rent3a and Rent3b serve as a link between splicing and NMD in the cytoplasm (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAGSChIP |
| recommended for detection of Rent1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 71-370 mapping near the N-terminus of Rent1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 71-370 mapping near the N-terminus of Rent1 of human origin |
| Entrez Gene | 5976 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human cerebellum tissue showing cytoplasmic staining of Purkinje cells.
Application
Western blot analysis of Rent1 expression in SK-N-SH whole cell lysate.
Application
Western blot analysis of Rent1 expression in Sol8 (A), SK-N-SH (B) and L6 (C) whole cell lysates.| Application Notes | recommended for detection of Rent1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
