Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts.Application
Western blot analysis of Cdc45 expression in non-transfected 293T: (A), human Cdc45 transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of Cdc45 expression in non-transfected 293T: (A), human Cdc45 transfected 293T: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of Cdc45 expression in non-transfected: (A) and human Cdc45 transfected: (B) 293T whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear staining of trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of Cdc45 expression in A-431 (A) and HeLa (B) nuclear extracts Antibodies tested include Cdc45 (C-20): (A) and Cdc45 (H-300): (B).| Product Name | Cdc45 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Cdc45 of human origin |
| Modification Notes | Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins (1). The cell division cycle (Cdc) genes are required at various points in the cell cycle (2). Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues (3). Cdc6 and Cdc45 are the mammalian homologs of S. cerevisiae Cdc6 and Cdc45, which are involved in the initiation of DNA replication (4,8). Cdc37 appears to facilitate Cdk4/cyclin D1 complex formation and has been shown to form a stable complex with HSP 90 (5). Cdc34, Cdc27 and Cdc16 function as ubiquitin-conjugating enzymes. Cdc34 is thought to be the structural and functional homolog of S. cerevisiae Cdc34, which is essential for the G1 to S phase transition (6). Cdc16 and Cdc27 are components of the APC (anaphase-promoting complex) which ubiquitinates cyclin B, resulting in cyclin B/Cdk complex degradation (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Cdc45 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Cdc45 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of Cdc45 of human origin |
| Entrez Gene | 8318 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic staining of cells in seminiferous ducts.
Application
Western blot analysis of Cdc45 expression in non-transfected 293T: (A), human Cdc45 transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of Cdc45 expression in non-transfected 293T: (A), human Cdc45 transfected 293T: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of Cdc45 expression in non-transfected: (A) and human Cdc45 transfected: (B) 293T whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue showing nuclear staining of trophoblastic cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of Cdc45 expression in A-431 (A) and HeLa (B) nuclear extracts Antibodies tested include Cdc45 (C-20): (A) and Cdc45 (H-300): (B).| Application Notes | recommended for detection of Cdc45 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
