Application
Western blot analysis of Chk1 expression in HeLa (A), K-562 (B) and NIH/3T3 (C) whole cell lysates.| Product Name | Chk1 Antibody (FL-476) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-476 representing full length Chk1 of human origin |
| Modification Notes | Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Chk1 of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-476 representing full length Chk1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-476 representing full length Chk1 of human origin |
| Entrez Gene | 1111 |
|---|
Application
Western blot analysis of Chk1 expression in HeLa (A), K-562 (B) and NIH/3T3 (C) whole cell lysates.| Application Notes | recommended for detection of Chk1 of mouse, rat, human and Xenopus origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
